
FAUSNAUGH, JAMES M., Gallagher Research and Development Co., Lakewood, CO
Detection of near surface soil alteration anomalies has been used for
many years as a hydrocarbon exploration tool. The alteration effects, when
reduced to the lowest common denominator, are attributed to hydrocarbon
microseepage and the presence of bacteria. Various microbial surveying
methods have been proposed and are being used today. These include the
detection of ethane, propane, or butane consuming bacteria. Microbial populations
increase over areas of active hydrocarbon microseepage, often forming an
apical anomaly. Areas of high hydrocarbon flux, such as faults or fractures,
yield a dramatic contrast relative to areas of minimal hydrocarbon seepage.
The proposed microbial method, which varies from others which rely
on specific hydrocarbon consuming bacteria, uses non-specific aerobic bacteria
that are ubiquitous in the near surface environment. As with other microbial
methods, the bacteria population increases over the hydrocarbon seepage
area. Bacteria counts are determined through a color change in the nutrient
media. Light, or white, indicates the absence of bacteria while dark red
indicates a significant number of microbes are present. Cultures are detected
within 48 to 72 hours after exposure to the soil sample. Numeric values
are assigned to each sample via image analysis of the exposed culture media.
Due to ease of sampling, minimal analytical setup, and rapid detection,
microbial surveys are an attractive alternative to other indirect near
surface hydrocarbon detection methods.